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Showing 1 to 12 of 751 entries
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CEK2, a Novel MAPK from Candida albicans Complement the Mating Defect of fus3/kss1 Mutant.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Chen J, Wang Q, Chen JY.
PMID: 12075459
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2000;32(3):299-304.

A novel MAPK(mitogen-activated protein kinase) gene, CEK2(Candida albicans extracellular signal-regulated kinase 2), was isolated by screening the Candida albicans library based on oligonucleotide probe hybridization and degenerated PCR. The CEK2 gene is 1 119 bp in length, and coding...

Cloning of the BPLA(2) Gene from Agkistrodon halys Pallas.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Pan H, Ou-Yang LL, Yang GZ, Zhou YC, Wu XF.
PMID: 12232583
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(6):579-582.

First strand cDNA synthesis was primed with synthetic oligonucleotide from total RNA extracted from the gland of the snake Agkistrodon halys Pallas. The pro-BPLA(2) gene was then amplified by PCR and cloned into the pBS-ks vector. Its nucleotide sequence...

Interaction between the Anion Exchange Protein and the Glucose Transport Protein in Human Red Cell Membranes.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Zhang ZH, Peng F, Li XQ, Zhou HQ, Hu XJ.
PMID: 12232595
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(6):665-670.

Cytochalasin B was used to inhibit the glucose transport through the glucose transport protein (GluT-1) in red cell membranes. It was observed that the anion transport was increased at the same time. The rate of anion transport was also...

Identification of the Photoaffinity-labeled Abscisic Acid Binding Proteins from Maize Root Microsome.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Wu ZY, Chen J, Zhu MJ.
PMID: 12232599
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(6):694-696.

ABA binding protein (ABA-BP) was solubilized with 0.2% (W/V) Triton X-100 from the microsomal membrane of maize root, with a specific binding activity of 0.56 pmol ABA/g FW. When ABA was cross-linked to its binding sites by irradiating the...

Protein Engineering of Insulin: [B9Glu, B10Asp] Human Insulin.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Liang ZH, Liu B, Tang YH, Feng YM.
PMID: 12232603
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(5):447-451.

B9Ser and B10His of the insulin B chain are substituted respectively by Glu and Asp using a gapped duplex DNA approach for site-directed mutagenesis. A mutant insulin-[B9Glu, B10Asp] human insulin was obtained. The receptor binding capacity of the mutant...

A Structure-function Analysis of the Human Ciliary Neurotrophic Factor.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

He C, Chen JY, Lu CL, Ao SZ.
PMID: 12232605
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(5):458-463.

The ciliary neurotrophic factor (CNTF) plays a very important role in the development and regeneration of the nervous system. In this study, the prediction of secondary structure and the hydrophobicity analysis of human CNTF were performed according to the...

The Expression of Human alpha(1,3) Fucosyltransferase in Human Liver Cancer by in situ Hybridization.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Fan J, Xie TP, Liu YJ, Hu Y, Gu TJ.
PMID: 12232620
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(5):568-571.

The increase of Lewis x and Sialyl Lewis x epitopes on the surface of cancer cells was known for decades. alpha(1,3) FuT is the key enzyme in the biosynthesis of these epitopes. The increase of the activity of this...

Changes in Glycosylation Alter the Affinity of the Human Transferrin for Its Placental Receptor.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Huang Y, Gu JX.
PMID: 12232626
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(4):352-357.

By using serial lectin-Sepharose affinity chromatography (ConA, DSA, and LCA), the transferrins carrying the biantennary complex type of oligosaccharides from sera of healthy individuals and those carrying multiantennary oligosaccharides from sera of pregnant women were purified. The two kinds...

The Fusion Expression of HBV preS Epitopes and the Core Antigen.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Li YC, Xu X, Chen ZY, Wang Y, Li GD.
PMID: 12232630
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(4):380-388.

The DNA fragments encoding the preS epitopes of HBV surface antigen were fused to the HBc gene and expressed in E. coli under the control of the tac promoter. The products were analyzed by ELISA and Western blotting, which...

Fluorescence Red-edge-excitation Effects of Human Serum Albumin.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Wu GM, Xu GJ.
PMID: 12232635
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(4):418-423.

We have studied the fluorescence red-edge-excitation effects of human serum albumin. Our results indicate that the red-edge-excitation effects of human serum albumin depend on the pH, temperature and concentration of the denaturant. The different red-edge-excitation effects are produced by...

Growth Promoting Activity of Chimeric Molecules of "Insulin-Insulin-like Growth Factor-I" .

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Zhang X, Tang YH, Feng YM, Jing NH.
PMID: 12232638
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(4):434-437.

The chimeric molecules of "Insulin-Insulin-like Growth Factor-I", Ins/IGFI(8) and Ins/IGF-I(11) were obtained by means of enzymatic semisynthesis. The growth promoting activity of these chimeric molecules were evaluated with a mouse mammary tumor derived cell line, GR2H6, and compared with...

The Study of a New Type of Membrane for Biosensor.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Wang SG, Ji XS, Yuan ZY.
PMID: 12232640
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(4):442-446.

Polyvinyl alcohol (PVA) was selected to develop a new type of membrane for biosensor. The PVA membrane was first activated by cyanogens bromide, followed by coupling with ethyl diamine. After treated by glutaraldehyde, glucose oxidase and lactate oxidase were...

Showing 1 to 12 of 751 entries